L-fucokinase/GDP-fucose pyrophos-phorylase (FKP) 

L-fucokinase/GDP-fucose pyrophos-phorylase (FKP) is an enzyme that catalyzes the chemical reaction. L-fucokinase/GDP-fucose pyrophos-phorylase (FKP) has substrate of L-fucose. L-fucokinase/GDP-fucose pyrophos-phorylase (FKP) induces L-fucose phosphorylation to form L-fucose-L-phosphate^[1].

Product Information
This product is expressed by recombinant Escherichia coli, N-terminal His Tagged, molecular weight 105 kDa, buffer components 20 mM Tris-HCl, 200 mM NaCl (pH 7.5), 0.5% Trehalose, can be used for the phosphorylation of fucose to generate Fuc-1-P, can also further catalyze Fuc-1-P to generate GDP-Fuc, as a donor for glycosyltransferase to transfer fucose, can be used as a tool enzyme for the synthesis of GDP-Fuc.

Instructions
1. Add substrate according to the reaction system, 5 mM MnCl2, and appropriate buffer salts (such as Tris-HCl) can be added to adjust the pH to 7.5-8.5, and add appropriate amount of FKP (according to reaction requirements)
2. After mixing, incubate the sample at 37℃ for 30-60 min. The recommended reaction buffer is Tris-HCl (pH8.0), and most common biological buffers are also applicable, such as Tris or PBS.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation. The mixture can be shaken at a low speed. Adding inorganic pyrophosphatase can increase the conversion rate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

37278-00-5

Liquid

Colorless to light yellow

[L-fucokinase/GDP-fucose pyrophos-phorylase (FKP)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zeitler R, et, al. Inhibition of L-fucokinase from rat liver by L-fucose analogues in vitro. J Enzyme Inhib. 1997 Feb;11(4):265-73.  [Content Brief]