2. Apoptosis
  3. Apoptosis Bcl-2 Family MDM-2/p53
  4. Lobetyol

Lobetyol is a natural compound that can be isolated from Lobelia chinensis. Lobetyol induces apoptosis and cell cycle arrest in MKN45 cells. Lobetyol shows anti-virus, anti-inflammation and anti-tumor activity. Lobetyol is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

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Lobetyol Chemical Structure

Lobetyol Chemical Structure

CAS No. : 136171-87-4

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Lobetyol Related Antibodies

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Description

Lobetyol is a natural compound that can be isolated from Lobelia chinensis. Lobetyol induces apoptosis and cell cycle arrest in MKN45 cells. Lobetyol shows anti-virus, anti-inflammation and anti-tumor activity[1][2]. Lobetyol is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC50 & Target

Bax

 

Bcl-2

 

In Vitro

Lobetyol (0-100 μg/mL, 0-48 h) leads to a dose-and time-dependent proliferation inhibition in MKN45 cells[1].
Lobetyol (0-100 μg/mL, 48 h) induces apoptosis and cell cycle arrest in a time- and dose-dependent manner in MKN45 cells[1].
Lobetyol (0-100 μg/mL, 48 h) increases the expression level of Bax, P53, and decreased the expression level of Bcl-2[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lobetyol Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MKN45 cells
Concentration: 0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL
Incubation Time: 12 h, 24 h, 48 h
Result: Showed a dose-and time-dependent proliferation inhibition in MKN45 cells, with an IC50 of 71.47 ± 4.29 μg/mL at 48 h. Exhibited more safety on L02 and HEK293 human normal cell lines.

Apoptosis Analysis[1]

Cell Line: MKN45 cells
Concentration: 0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL
Incubation Time: 48 h
Result: Increased the apoptotic population in MKN45 cells in a dose-dependent manner. Flow cytometic assay of Annexin-V/PI revealed apoptotic populations under increasing dose of lobetyol ranging from 0 μg/mL, 50 μg/mL, 75 μg/mL, to 100 μg/mL are 5.5%, 13.74%, 27.32% to 31.57%, respectively.

Western Blot Analysis[1]

Cell Line: MKN45 cells
Concentration: 0 μg/mL, 50 μg/mL, 75 μg/mL, 100 μg/mL
Incubation Time: 12 h, 24 h, 48 h
Result: Increased the expression level of Bax, P53, and decreased the expression level of Bcl-2. The release of cytochrome c was detected, followed by caspase-9 and -3 activations.
In Vivo

Lobetyol (0-10 mg/kg, Once per 3 days) suppresses tumor growth in MKN45 nude models[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/C nude mice (6 weeks-old, injected intraperitoneally with MKN45 cells)[1]
Dosage: 0, 2, 5, 10 mg/kg
Administration: Once per 3 days
Result: Suppressed tumor growth in MKN45 nude models. The protein levels of Ki67 in MKN45 cells were significantly decreased. Meanwhile, cleaved-caspase-3 positive MKN45 cells were found increased with the tendency of lobetyol concentrations in the study in vivo.
Molecular Weight

234.29

Formula

C14H18O3
CAS No.
SMILES

C/C=C/C#CC#CC(O)C(O)/C=C/CCCO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Lobetyol Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lobetyol136171-87-4ApoptosisBcl-2 FamilyMDM-2/p53MKN45Cell cycle arrestMAPKInhibitorinhibitorinhibit

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