Macrophage-activating lipopeptide 2 (Synonyms: MALP-2)

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Macrophage-activating lipopeptide 2 (MALP-2) is an agonist of Toll like receptors TLR-2/TLR-6. Macrophage-activating lipopeptide-2 can enhance endothelial nitric oxide synthase (eNOS) phosphorylation and endothelial cell release of NO, thereby improving vasodilation. Macrophage-activating lipopeptide-2 can enhance endothelial adhesion of white blood cells and improve perfusion recovery and collateral growth in the hind limbs of hypercholesterolemic Apoe deficient mice undergoing experimental femoral artery ligation (FAL).

For research use only. We do not sell to patients.

Macrophage-activating lipopeptide 2 Chemical Structure

CAS No. : 250718-44-6

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nNOS iNOS eNOS

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Purity & Documentation
References
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Description

IC₅₀ & Target

TLR2

TLR6

In Vitro

Macrophage-activating lipopeptide 2 (1 µg/mL, 24 h) can promote the expression of major endothelial adhesion molecules and leukocyte integrin receptors, thereby enhancing leukocyte endothelial adhesion^[1].
Macrophage-activating lipopeptide 2 (1 µg/mL, 2 h) can promote the phosphorylation of protein kinase B and eNOS in endothelial MyEnd cells, thereby promoting NO release^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1].

Cell Line:	Endothelial MyEnd cells
Concentration:	1 µg/mL
Incubation Time:	24 h
Result:	Caused a strong transient increase in mRNA levels of vascular cell adhesion molecule-1 (Vcam-1) after 1 hour, and also increased the levels of other major endothelial adhesion molecules, namely intercellular adhesion molecule-1 (Icam-1), E-selectin, and P-selectin, between 1-3 hours.

Western Blot Analysis^[1].

Cell Line:	Endothelial MyEnd cells
Concentration:	1 µg/mL
Incubation Time:	2 h
Result:	Promoted phosphorylation of protein kinase B and eNOS.

In Vivo

Macrophage-activating lipopeptide 2 (1 µg in 125 µL PBS; once daily; 10 days; i.v.) can improve hind limb perfusion recovery and collateral growth in Apoe deficient mice with experimental femoral artery ligation (FAL) and hypercholesterolemia^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6-10 weeks old male C57BL/6 Hypercholesterolemic Apoe-deficient mice^[1].
Dosage:	1 µg in 125 µL PBS
Administration:	Intravenous injection (i.v.); once daily; 10 days
Result:	Improved blood flow recovery and increased the growth of pre-existing collateral arteries in the upper and hind limbs, as well as the proliferation of endothelial cells and accumulation of peri collagenous macrophages in the collateral walls.

Molecular Weight

2135.56

Formula

C₉₉H₁₆₇N₁₉O₃₀S

CAS No.

250718-44-6

Sequence

{S-[(2R)-2,3-bis[(1-oxohexadecyl)oxy]propyl]Cys}-Gly-Asn-Asn-Asp-Glu-Ser-Asn-Ile-Ser-Phe-Lys-Glu-Lys

Sequence Shortening

{S-[(2R)-2,3-bis[(1-oxohexadecyl)oxy]propyl]Cys}-GNNDESNISFKEK

SMILES

O=C(N[C@@H](CCCCN)C(N[C@@H](CCC(O)=O)C(N[C@H](C(O)=O)CCCCN)=O)=O)[C@@H](NC([C@H](CO)NC([C@H]([C@@H](C)CC)NC([C@H](CC(N)=O)NC([C@H](CO)NC([C@H](CCC(O)=O)NC([C@H](CC(O)=O)NC([C@H](CC(N)=O)NC([C@H](CC(N)=O)NC(CNC([C@@H](N)CSC[C@H](OC(CCCCCCCCCCCCCCC)=O)COC(CCCCCCCCCCCCCCC)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)CC1=CC=CC=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Troidl K, et al. The Lipopeptide MALP-2 Promotes Collateral Growth. Cells. 2020 Apr 16;9(4):997. [Content Brief]

Macrophage-activating lipopeptide 2 Related Classifications

Cardiovascular Disease

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Macrophage-activating lipopeptide 2250718-44-6MALP-2MALP2MALP 2Toll-like Receptor (TLR)NO SynthaseNitric oxide synthasesNOSToll-like receptorMyEnd cellendothelial adhesioncollateral growthInhibitorinhibitorinhibit

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