Malic dehydrogenase, microorganism  (Synonyms: MDH; EC 1.1.1.37)

Malate dehydrogenase (EC 1.1.1.37) (MDH) catalyzes the mutual conversion of oxaloacetate and malate, and is associated with the oxidation/reduction of dinucleotide coenzymes^[1].

Molecular weight: approx: 35,000 (by gel filtration)
Michaelis constants: 1.5×10^-6 M (NADH, 37°C),1.9×10^-6 M (Oxaloacetate, 37°C)
Optimu M pH: 7.5-8.0
Optimu M temperature: 60°C
pH Stability: 3.0-9.0 (37°C, 30 min)
Thermal stability: below 65℃ (pH 7.5, 30 min)
Solubility: MDH was dissolved at concentration of 20 U/mL in 100 mM, pH 7.5,Tris-HCl buffer.
Product Information
Molecular weight: about 35,000
Michaelis constant: 1.5×10^-6 M (NADH, 37°C), 1.9×10^-6 M (oxaloacetate, 37°C)
Optimum pH: 7.5-8.0
Optimum temperature: 60°C
pH stability: 3.0-9.0 (37°C, 30 minutes)
Thermal stability: below 65°C (pH 7.5, 30 minutes)

Instructions
Dissolved in 100 mM Tris-HCl, pH 7.5 at a concentration of 20 U/mL. Or dissolve in water or buffer according to specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9001-64-3

Solid

White to off-white

[Malic dehydrogenase, microorganism]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Goward CR, et al. Malate dehydrogenase: a model for structure, evolution, and catalysis. Protein Sci. 1994 Oct;3(10):1883-8.  [Content Brief]