Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) (Synonyms: Manganese(Ⅱ) chloride (tetrahydrate), molecular biology grade,≥99.0% (KT))

Cat. No.: HY-109521A Purity: 99.08%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) is an orally active and a blood-brain barrier penetrant compound. It affects the activities of multiple enzymes in cells, such as regulating the activities of histone acetyltransferase (HAT) and histone deacetylase (HDAC), thereby affecting gene expression. It has multiple activities such as neurotoxicity, embryotoxicity, and reproductive toxicity. It is currently mainly used in neurodegenerative diseases and toxicology research.

For research use only. We do not sell to patients.

Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) Chemical Structure

CAS No. : 13446-34-9

Size	Stock	Quantity
Free Sample (0.1 - 0.5 mg)	Apply Now
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Histone Acetyltransferase Isoform Specific Products:

View All Isoforms

CBP/p300 GCN5/PCAF TIP60 MOZ/MORF HBO1

View All HDAC Isoform Specific Products:

View All Isoforms

HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Manganese chloride tetrahydrate, molecular biology grade,≥99.0% (KT) (15-25 μM) shows cytotoxicity and causes chromosomal aberrations as well as DNA damage in cultured human lymphocytes^[1].
Manganese chloride (300 μM; 3-24 h) tetrahydrate, molecular biology grade, ≥99.0% (KT) can significantly inhibit the acetylation of histones H3 and H4 and change the activities and expression levels of HAT and HDAC in PC12 cells and SH-SY5Y cells^[2].
Manganese chloride (0.1-10 mM; 5 days) tetrahydrate, molecular biology grade,≥99.0% (KT) reduces cell viability and inhibits the differentiation of cells into cartilage nodules in micromass cultures of mouse embryonic limb bud cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	PC12 cells
Concentration:	300 μM
Incubation Time:	3 h, 6 h, 12 h, 24 h
Result:	Reduced the acetylation of histone H3 and H4. Increased the activity of HDAC and Decreased that of HAT.

In Vivo

Manganese chloride (1000 - 8000 mg/l; ingested through drinking water, p.o.; for 12 weeks) tetrahydrate, molecular biology grade, ≥99.0% (KT) reduces the fertility of male Swiss mice and decreases the number of implantation sites and viable fetuses in female Swiss mice at a high concentration (8000 mg/l)^[4].
Manganese chloride (0-100 mg/kg; s.c.; single-dose or multiple-dose; 1 day or 7 days) tetrahydrate, molecular biology grade, ≥99.0% (KT) significantly increases the manganese concentration in the basal ganglia and causes a decrease in horizontal movement in C57BL/6 mice^[5].
Manganese chloride (0-50 mg/kg; p.o.; once a day; 21 days) tetrahydrate, molecular biology grade, ≥99.0% (KT) reduces body weight gain and increases the amplitude of the acoustic startle response in neonatal CD rats, and increases striatal, cerebellar and brain residue manganese concentrations in adult CD rats at high doses^[6].
Manganese chloride (16 mg/kg/day; i.v.; once a day; 4 days) tetrahydrate, molecular biology grade, ≥99.0% (KT) leads to the death or moribund sacrifice of male beagle dogs, with severe hepatotoxicity, hypotension and reflex tachycardia^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 male retired breeder mice (7-9 months old), model for studying manganese-induced neurotoxicity and its relation to Parkinson's disease^[5]
Dosage:	50, 100 mg/kg (dissolved in distilled water)
Administration:	Subcutaneous injection, single-dose or three-dose (for 7-day survival time), 1 day or 7 days
Result:	Increased striatal manganese concentration by 105% in 1 day at a single 100 mg/kg dose. Increased striatal manganese concentration by 421% and 647% in 1 day at multiple doses of 50 and 100 mg/kg, respectively. Decreased horizontal movement at 50, 100 mg/kg (single-dose) and 100 mg/kg (multiple-dose).

Animal Model:	Male beagle dogs (about 8-month-old), model for evaluating acute toxicologic potential of manganese chloride^[7]
Dosage:	16 mg/kg/day (prepared as a 2 mg/mL solution in 0.9% sodium chloride for injection)
Administration:	Intravenous infusion, once a day, for 4 days
Result:	Showed clinical signs of toxicity such as loss of appetite, reduction in blood pressure with reflex tachycardia, and a marked increase in liver enzymes. Confirmed severe hepatotoxicity by gross and histopathologic evaluations.

Molecular Weight

197.91

Formula

Cl₂H₈MnO₄

CAS No.

13446-34-9

Appearance

Solid

Color

Off-white to pink

SMILES

[MnCl2 ?4H2O]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 1666.67 mg/mL (8421.35 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.0528 mL	25.2640 mL	50.5280 mL
5 mM	1.0106 mL	5.0528 mL	10.1056 mL
10 mM	0.5053 mL	2.5264 mL	5.0528 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.08%

Select Batch:

Data Sheet (277 KB) SDS (808 KB)

COA (244 KB)

Handling Instructions (2659 KB)

References

[1]. Lima PD, et al. Genotoxic and cytotoxic effects of manganese chloride in cultured human lymphocytes treated in different phases of cell cycle. Toxicol In Vitro. 2008 Jun;22(4):1032-7. [Content Brief]

[2]. Guo Z, et al. Manganese chloride induces histone acetylation changes in neuronal cells: Its role in manganese-induced damage. Neurotoxicology. 2018 Mar;65:255-263. [Content Brief]

[3]. Doyle D, et al. Inhibition of cell differentiation by manganese chloride in micromass cultures of mouse embryonic limb bud cells. Toxicol In Vitro. 2002 Apr;16(2):101-6. [Content Brief]

[4]. Elbetieha A, et al. Effects of long-term exposure to manganese chloride on fertility of male and female mice. Toxicol Lett. 2001 Mar 8;119(3):193-201. [Content Brief]

[5]. Dodd CA, et al. Basal Ganglia accumulation and motor assessment following manganese chloride exposure in the C57BL/6 mouse. Int J Toxicol. 2005 Nov-Dec;24(6):389-97. [Content Brief]

[6]. Dorman DC, et al. Neurotoxicity of manganese chloride in neonatal and adult CD rats following subchronic (21-day) high-dose oral exposure. J Appl Toxicol. 2000 May-Jun;20(3):179-87. [Content Brief]

[7]. Khan KN, et al. Toxicity of subacute intravenous manganese chloride administration in beagle dogs. Toxicol Pathol. 1997 Jul-Aug;25(4):344-50. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.0528 mL	25.2640 mL	50.5280 mL	126.3200 mL
	5 mM	1.0106 mL	5.0528 mL	10.1056 mL	25.2640 mL
	10 mM	0.5053 mL	2.5264 mL	5.0528 mL	12.6320 mL
	15 mM	0.3369 mL	1.6843 mL	3.3685 mL	8.4213 mL
	20 mM	0.2526 mL	1.2632 mL	2.5264 mL	6.3160 mL
	25 mM	0.2021 mL	1.0106 mL	2.0211 mL	5.0528 mL
	30 mM	0.1684 mL	0.8421 mL	1.6843 mL	4.2107 mL
	40 mM	0.1263 mL	0.6316 mL	1.2632 mL	3.1580 mL
	50 mM	0.1011 mL	0.5053 mL	1.0106 mL	2.5264 mL
	60 mM	0.0842 mL	0.4211 mL	0.8421 mL	2.1053 mL
	80 mM	0.0632 mL	0.3158 mL	0.6316 mL	1.5790 mL
	100 mM	0.0505 mL	0.2526 mL	0.5053 mL	1.2632 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT) Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Manganese chloride (tetrahydrate), molecular biology grade,≥99.0% (KT)13446-34-9Manganese(Ⅱ) chloride (tetrahydrate), molecular biology grade,≥99.0% (KT)Biochemical Assay ReagentsHistone AcetyltransferaseHDACHATsHATHistone deacetylasesbiochemical assayreagentInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *