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  3. High-performance liquid chromatographic determination of pholedrine in human serum using ion-pair extraction and amperometric detection

High-performance liquid chromatographic determination of pholedrine in human serum using ion-pair extraction and amperometric detection

  • J Chromatogr B Biomed Sci Appl. 1999 Apr 16;726(1-2):309-12. doi: 10.1016/s0378-4347(99)00068-7.
G P Peinhardt 1
Affiliations

Affiliation

  • 1 Institute of Pharmaceutical Chemistry, Faculty of Pharmacy, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.
PMID: 10348201 DOI: 10.1016/s0378-4347(99)00068-7
Abstract

The method for the quantitation of pholedrine in human serum involves extraction of the sample with benzene utilizing an ion-pairing reagent, bis(2-ethylhexyl)phosphoric acid, and re-extraction into diluted phosphoric acid. Analysis is carried out on a ODS reversed-phase column with heptanesulfonate as the ion-pairing reagent. The procedure allows quantitation at the lower nanogram level and is useful for pharmacokinetic investigations.

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