2. Academic Validation
  3. Isolation and characterization of a novel TP53-inducible gene, TP53TG5, which suppresses growth and shows cell cycle-dependent transition of expression

Isolation and characterization of a novel TP53-inducible gene, TP53TG5, which suppresses growth and shows cell cycle-dependent transition of expression

  • Genes Chromosomes Cancer. 2000 Apr;27(4):345-52. doi: 10.1002/(sici)1098-2264(200004)27:4<345::aid-gcc2>3.0.co;2-3.
S Isaka 1,  Y Takei,  T Tokino,  K Koyama,  Y Miyoshi,  M Suzuki,  E Takahashi,  C Azuma,  Y Murata,  Y Nakamura
Affiliations

Affiliation

  • 1 Division of Clinical Genetics, Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Osaka, Japan.
PMID: 10719363 DOI: 10.1002/(sici)1098-2264(200004)27:4<345::aid-gcc2>3.0.co;2-3
Abstract

Through a strategy of direct cloning of TP53-binding DNA sequences from human genome DNA, we have identified a novel TP53-target gene, termed TP53TG5 (TP53-target gene 5). This gene, localized to chromosome band 20q13.1 by fluorescence in situ hybridization, encodes a 290-amino-acid peptide with no significant homology with any known proteins in the public database. A colony-formation assay using human glioblastoma cell line T98G, which lacks wild-type TP53 and expresses no endogenous TP53TG5, revealed a growth-suppressive effect of the TP53TG5 gene product. Furthermore, immunohistochemical studies, following transfection of T98G with plasmid designed to express green fluorescent protein-fused TP53TG5, revealed cell cycle-dependent intracellular localization of this protein. Our results suggest that functional studies of TP53TG5 may provide new insights into the complex physiological activities of TP53.

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