2. Academic Validation
  3. Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cells

Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cells

  • Nat Cell Biol. 2001 Jun;3(6):567-72. doi: 10.1038/35078543.
S X Lin 1,  B Grant,  D Hirsh,  F R Maxfield
Affiliations

Affiliation

  • 1 Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA.
PMID: 11389441 DOI: 10.1038/35078543
Abstract

RME-1 is an Eps15-homology (EH)-domain protein that was identified in a genetic screen for endocytosis genes in Caenorhabditis elegans. When expressed in a CHO cell line, the worm RME-1 protein and a mouse homologue are both associated with the endocytic recycling compartment. Here we show that expression of a dominant-negative construct with a point mutation near the EH domain results in redistribution of the endocytic recycling compartment and slowing down of Transferrin Receptor recycling. The delivery of a TGN38 chimaeric protein to the trans-Golgi network is also slowed down. The function of Rme-1 in endocytic recycling is evolutionarily conserved in metazoans as shown by the protein's properties in C. elegans.

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