1. Academic Validation
  2. Human thymic stromal lymphopoietin preferentially stimulates myeloid cells

Human thymic stromal lymphopoietin preferentially stimulates myeloid cells

  • J Immunol. 2001 Jul 1;167(1):336-43. doi: 10.4049/jimmunol.167.1.336.
P A Reche 1 V Soumelis D M Gorman T Clifford Liu Mr M Travis S M Zurawski J Johnston Y J Liu H Spits R de Waal Malefyt R A Kastelein J F Bazan
Affiliations

Affiliation

  • 1 DNAX Research Institute, Palo Alto, CA 94304, USA.
Abstract

The sequence of a novel hemopoietic cytokine was discovered in a computational screen of genomic databases, and its homology to mouse thymic stromal lymphopoietin (TSLP) suggests that it is the human orthologue. Human TSLP is proposed to signal through a heterodimeric receptor complex that consists of a new member of the hemopoietin family termed human TSLP receptor and the IL-7R alpha-chain. Cells transfected with both receptor subunits proliferated in response to purified, recombinant human TSLP, with induced phosphorylation of STAT3 and STAT5. Human TSLPR and IL-7Ralpha are principally coexpressed on monocytes and dendritic cell populations and to a much lesser extent on various lymphoid cells. In accord, we find that human TSLP functions mainly on myeloid cells; it induces the release of T cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells, as evidenced by the strong induction of the costimulatory molecules CD40 and CD80 and the enhanced capacity to elicit proliferation of naive T cells.

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