1. Academic Validation
  2. Titin mutations as the molecular basis for dilated cardiomyopathy

Titin mutations as the molecular basis for dilated cardiomyopathy

  • Biochem Biophys Res Commun. 2002 Feb 22;291(2):385-93. doi: 10.1006/bbrc.2002.6448.
Manatsu Itoh-Satoh 1 Takeharu Hayashi Hirofumi Nishi Yoshinori Koga Takuro Arimura Takeshi Koyanagi Megumi Takahashi Shigeru Hohda Kazuo Ueda Tatsuhito Nouchi Michiaki Hiroe Fumiaki Marumo Tsutomu Imaizumi Michio Yasunami Akinori Kimura
Affiliations

Affiliation

  • 1 Department of Molecular Pathogenesis, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kandasurugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Abstract

Dilated cardiomyopathy (DCM) is a heterogeneous cardiac disease characterized by ventricular dilatation and systolic dysfunction. Recent genetic studies have revealed that mutations in genes for cardiac sarcomere components lead to DCM. The cardiac sarcomere consists of thick and thin filaments and a giant protein, titin. Because one of the loci of familial DCM was mapped to the region of the titin gene, we searched for titin mutations in the patients and identified four possible disease-associated mutations. Two mutations, Val54Met and Ala743Val, were found in the Z-line region of titin and decreased binding affinities of titin to Z-line proteins T-cap/telethonin and alpha-actinin, respectively, in yeast two-hybrid assays. The other two mutations were found in the cardiac-specific N2-B region of titin and one of them was a nonsense mutation, Glu4053ter, presumably encoding for a truncated nonfunctional molecule. These observations suggest that titin mutations may cause DCM in a subset of the patients.

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