2. Academic Validation
  3. Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage-gated K(+) channel Kv2.1

Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage-gated K(+) channel Kv2.1

  • FEBS Lett. 2002 Feb 13;512(1-3):230-4. doi: 10.1016/s0014-5793(02)02267-6.
Yorikata Sano 1,  Shinobu Mochizuki,  Akira Miyake,  Chika Kitada,  Kohei Inamura,  Hiromichi Yokoi,  Katsura Nozawa,  Hitoshi Matsushime,  Kiyoshi Furuichi
Affiliations

Affiliation

  • 1 Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, Ibaraki 305-8585, Tsukuba, Japan. sano.yorikata@yamanouchi.co.jp
PMID: 11852086 DOI: 10.1016/s0014-5793(02)02267-6
Abstract

We report identification and characterization of Kv6.3, a novel member of the voltage-gated K(+) channel. Reverse transcriptase-polymerase chain reaction analysis indicated that Kv6.3 was highly expressed in the brain. Electrophysiological studies indicated that homomultimeric Kv6.3 did not yield a functional voltage-gated ion channel. When Kv6.3 and Kv2.1 were co-expressed, the heteromultimeric channels displayed the decreased rate of deactivation compared to the homomultimeric Kv2.1 channels. Immunoprecipitation studies indicated that Kv6.3 bound with Kv2.1 in co-transfected cells. These results indicate that Kv6.3 is a novel member of the voltage-gated K(+) channel which functions as a modulatory subunit.

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