2. Academic Validation
  3. Dexamethasone treatment specifically increases the basal proton conductance of rat liver mitochondria

Dexamethasone treatment specifically increases the basal proton conductance of rat liver mitochondria

  • FEBS Lett. 2003 Apr 24;541(1-3):75-9. doi: 10.1016/s0014-5793(03)00307-7.
Damien Roussel 1,  Jean-François Dumas,  Antoine Augeraud,  Olivier Douay,  Françoise Foussard,  Yves Malthiéry,  Gilles Simard,  Patrick Ritz
Affiliations

Affiliation

  • 1 Laboratoire de biochimie et de biologie moléculaire, INSERM EMI-U 00.18, Angers, France.
PMID: 12706822 DOI: 10.1016/s0014-5793(03)00307-7
Abstract

We investigated the role that mitochondrial proton leak may play in the glucocorticoid-induced hypermetabolic state. Sprague-Dawley rats were injected with dexamethasone over a period of 5 days. Liver mitochondria and gastrocnemius subsarcolemmal and intermyofibrillar mitochondria were isolated from dexamethasone-treated, pair-fed and control rats. Respiration and membrane potential were measured simultaneously using electrodes sensitive to oxygen and to the potential-dependent probe triphenylmethylphosphonium, respectively. Five days of dexamethasone injection resulted in a marked increase in the basal proton conductance of liver mitochondria, but not in the muscle mitochondrial populations. This effect would have a modest impact on energy expenditure in rats.

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