2. Academic Validation
  3. Apoptotic activities of C2-ceramide and C2-dihydroceramide homologues against HL-60 cells

Apoptotic activities of C2-ceramide and C2-dihydroceramide homologues against HL-60 cells

  • Bioorg Med Chem. 2003 Jul 3;11(13):2723-8. doi: 10.1016/s0968-0896(03)00228-1.
Keiji Shikata 1,  Hayato Niiro,  Hideki Azuma,  Kenji Ogino,  Taro Tachibana
Affiliations

Affiliation

  • 1 Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.
PMID: 12788346 DOI: 10.1016/s0968-0896(03)00228-1
Abstract

The apoptotic activities of non-natural ceramide homologues, C2-homo-ceramide, C2-homo-dihydroceramide, C2-bishomo-ceramide and C2-bishomo-dihydroceramide, were examined using human leukemia HL-60 cells. The apoptotic activity was in order of C2-ceramide>C2-homo-ceramide approximately C2-bishomo-ceramide and the activities of the L-erythro- and D-erythro-ceramide homologues were similar. The morphological features of the cells, DNA fragmentations, proteolytic processing of pro-caspase-3 and the cleavage of PARP as the result of treatments with these homologues indicated that cell death was induced by Apoptosis.

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