2. Academic Validation
  3. Bioactive novel polyphenols from the fruit of Manilkara zapota (Sapodilla)

Bioactive novel polyphenols from the fruit of Manilkara zapota (Sapodilla)

  • J Nat Prod. 2003 Jul;66(7):983-6. doi: 10.1021/np020576x.
Jun Ma 1,  Xiao-Dong Luo,  Petr Protiva,  Hui Yang,  Cuiying Ma,  Margaret J Basile,  I Bernard Weinstein,  Edward J Kennelly
Affiliations

Affiliation

  • 1 Department of Biological Sciences, Lehman College and The Graduate Center, The City University of New York, 250 Bedford Park Boulevard West, Bronx, New York 10468, USA.
PMID: 12880319 DOI: 10.1021/np020576x
Abstract

Activity-guided fractionation of a methanol extract from the fruit of Manilkara zapota cv. Tikal resulted in the isolation of two new Antioxidants, methyl 4-O-galloylchlorogenate (1) and 4-O-galloylchlorogenic acid (2), along with eight known polyphenolic Antioxidants, namely, methyl chlorogenate (3), dihydromyricetin (4), quercitrin (5), myricitrin (6), (+)-catechin (7), (-)-epicatechin (8), (+)-gallocatechin (9), and gallic acid (10). Of the 10 Polyphenols, 1 showed the highest antioxidant activity (IC(50) = 12.9 microM) in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical assay and displayed cytotoxicity in the HCT-116 and SW-480 human colon Cancer cell lines with IC(50) values of 190 and 160 microM, respectively. Compound 2 showed high antioxidant activity (IC(50) = 23.5 microM) in the DPPH free-radical assay and displayed cytotoxicity in the HCT-116 and SW-480 human colon Cancer cell lines with IC(50) values of 154 and 134 microM, respectively.

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