2. Academic Validation
  3. Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant protein

Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant protein

  • Cell Mol Biol Lett. 2003;8(3):783-92.
Małgorzata Palczewska 1,  Gyula Batta,  Patrick Groves
Affiliations

Affiliation

  • 1 Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
PMID: 12949617
Abstract

Pichia pastoris secretes few Native Proteins. However, the more than 1 g l(-1)of extracellularly expressed mannan interfered with the purification of our extracellularly expressed, non-glycosylated recombinant protein. Concanavalin A-agarose removed more than 95% of the unwanted mannan as monitored by phenol reaction. A(13)C-based NMR assay confirmed this improvement. Concanavalin A-agarose can assist the purification of extracellular expressed, non-glycosylated proteins from yeasts.

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