Alpha-naphthoflavone induces vasorelaxation through the induction of extracellular calcium influx and NO formation in endothelium

The effect of alpha-naphthoflavone (alpha-NF) on vascular function was studied in isolated ring segments of the rat thoracic aorta and in primary cultures of human umbilical vein endothelial cells (HUVECs). alpha-NF induced concentration-dependent relaxation of the phenylephrine-precontracted aorta endothelium-dependently and -independently at lower and higher concentrations, respectively. The cGMP, but not cAMP, content was increased significantly in alpha-NF-treated aorta. Pretreatment with N(omega)-nitro- l-arginine methyl ester (L-NAME) or methylene blue attenuated both alpha-NF induced vasorelaxation and the increase of cGMP content significantly. The increase of cGMP content induced by alpha-NF was also inhibited by chelating extracellular CA(2+) with EGTA. These results suggest that the endothelium-dependent vasorelaxation induced by alpha-NF is mediated most probably through CA(2+)-dependent activation of NO Synthase and guanylyl cyclase. In HUVECs, alpha-NF induced concentration-dependent formation of NO and CA(2+) influx. alpha-NF-induced NO formation was abolished by removal of extracellular CA(2+) and by pretreatment with the CA(2+) channel blockers SKF 96365 and Ni(2+), but not by the L-type CA(2+) channel blocker verapamil. The CA(2+) influx, as measured by (45)CA(2+) uptake, induced by alpha-NF was also inhibited by SKF 96365 and Ni(2+). Our data imply that alpha-NF, at lower concentrations, induces endothelium-dependent vasorelaxation by promoting extracellular CA(2+) influx in endothelium and the activation of the NO-cGMP pathway.