2. Academic Validation
  3. Molecular requirements for the regulation of the renal outer medullary K(+) channel ROMK1 by the serum- and glucocorticoid-inducible kinase SGK1

Molecular requirements for the regulation of the renal outer medullary K(+) channel ROMK1 by the serum- and glucocorticoid-inducible kinase SGK1

  • Biochem Biophys Res Commun. 2003 Nov 21;311(3):629-34. doi: 10.1016/j.bbrc.2003.10.037.
Monica Palmada 1,  Hamdy M Embark,  Chris Yun,  Christoph Böhmer,  Florian Lang
Affiliations

Affiliation

  • 1 Department of Physiology I, University of Tübingen, Gmelinstr 5, 72076 Tübingen, Germany.
PMID: 14623317 DOI: 10.1016/j.bbrc.2003.10.037
Abstract

The serum- and glucocorticoid- inducible kinase SGK1 stimulates the renal outer medullary K(+) channel ROMK1 in the presence of the Na(+)/H(+) exchanger regulating factor NHERF2. SGK1/NHERF2 are effective through enhancement of ROMK1 abundance within the cell membrane. The present study aims to define the molecular requirements for the interaction of ROMK1 with SGK1/NHERF2. Pull down assays reveal that SGK1 interacts with NHERF2 through the second PDZ domain of NHERF2. According to chemiluminescence and electrophysiology, deletion of the second PDZ domain of NHERF2 or the putative PDZ binding motif on ROMK1 abrogates the stimulating effect of SGK1 on ROMK1 protein abundance in the plasma membrane and K(+) current.

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