2. Academic Validation
  3. Agrocybin, an antifungal peptide from the edible mushroom Agrocybe cylindracea

Agrocybin, an antifungal peptide from the edible mushroom Agrocybe cylindracea

  • Peptides. 2005 Feb;26(2):191-6. doi: 10.1016/j.peptides.2004.09.011.
Patrick H K Ngai 1,  Zheng Zhao,  T B Ng
Affiliations

Affiliation

  • 1 Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
PMID: 15629530 DOI: 10.1016/j.peptides.2004.09.011
Abstract

An Antifungal peptide with a molecular mass of 9 kDa was isolated from fresh fruiting bodies of the mushroom Agrocybe cylindracea. The isolation procedure comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (FPLC) on Mono S, and FPLC-gel filtration on a Superdex 75 column. The Antifungal peptide, designated as agrocybin, was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and Mono S. Agrocybin exerted Antifungal activity against several Fungal species but lacked inhibitory activity against bacteria when tested up to 300 microM. The activity of HIV-1 Reverse Transcriptase was attenuated in the presence of agrocybin. It exhibited weaker mitogenic activity than Con A on isolated murine splenocytes, but was devoid of antiproliferative activity on Hep G2 (hepatoma) cells when tested at 110 microM.

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