2. Academic Validation
  3. Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing

Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing

  • Nat Genet. 2005 Mar;37(3):254-64. doi: 10.1038/ng1516.
K N Harikrishnan 1,  Maggie Z Chow,  Emma K Baker,  Sharmistha Pal,  Sahar Bassal,  Daniella Brasacchio,  Li Wang,  Jeff M Craig,  Peter L Jones,  Saïd Sif,  Assam El-Osta
Affiliations

Affiliation

  • 1 The Alfred Medical Research and Education Precinct, Baker Medical Research Institute, Epigenetics in Human Health and Disease Laboratory, Second Floor, Commercial Road, Prahran, Victoria 3181, Australia.
PMID: 15696166 DOI: 10.1038/ng1516
Abstract

Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in Cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.

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