2. Academic Validation
  3. A new Hypocrea strain producing harzianum A cytotoxic to tumour cell lines

A new Hypocrea strain producing harzianum A cytotoxic to tumour cell lines

  • Lett Appl Microbiol. 2005;40(6):497-503. doi: 10.1111/j.1472-765X.2005.01719.x.
H B Lee 1,  Y Kim,  H Z Jin,  J J Lee,  C-J Kim,  J Y Park,  H S Jung
Affiliations

Affiliation

  • 1 Department of Biological Sciences, Seoul National University, Seoul, Korea.
PMID: 15892749 DOI: 10.1111/j.1472-765X.2005.01719.x
Abstract

Aims: To identify a new Fungal strain, Hypocrea sp. F000527 producing a trichothecene metabolite, harzianum A, and to evaluate its cytotoxicity to tumour cell lines.

Methods and results: A Fungal strain, F000527, with cytotoxic activity was identified as a new Hypocrea strain based on morphological characteristics and internal transcribed spacers rDNA sequence data. Harzianum A was isolated from wheat bran culture by 50% acetone extraction, silica gel chromatography, Sephadex LH-20 chromatography and HPLC. The chemical structures were determined by ESI- or HRFAB-MS and (1)H and (13)C-NMR analyses. Harzianum A showed cytotoxicity to HT1080 and HeLa cell lines with IC(50) value of 0.65 and 5.07 Łg ml(-1) respectively.

Conclusions: Harzianum A with a chemical formula of C(23)H(28)O(6) was isolated from a new Hypocrea strain and showed moderate to strong cytotoxicity to human Cancer cell lines.

Significance and impact of the study: This is the first report of the production of cytotoxic harzianum A by a new Hypocrea strain.

Figures
Products