2. Academic Validation
  3. Osteopontin: a rapid and sensitive response to dioxin exposure in the osteoblastic cell line UMR-106

Osteopontin: a rapid and sensitive response to dioxin exposure in the osteoblastic cell line UMR-106

  • Biochem Biophys Res Commun. 2006 Mar 3;341(1):116-20. doi: 10.1016/j.bbrc.2005.12.158.
Carolina Wejheden 1,  Sara Brunnberg,  Annika Hanberg,  P Monica Lind
Affiliations

Affiliation

  • 1 Division of Biochemical Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden. Carolina.Wejheden@ki.se
PMID: 16414014 DOI: 10.1016/j.bbrc.2005.12.158
Abstract

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is an endocrine disrupting environmental pollutant that, among Other effects, affects bone tissue. TCDD modulates the transcription of various genes, e.g., CYP1A1, and the present study is a part of a project aiming at developing an in vitro model system for identifying biomarkers specific for dioxin-induced effects in osteoblasts. Osteopontin (OPN) is an adhesion protein, suggested to be important in bone remodeling and our results indicate that TCDD down-regulates the transcription of OPN in the osteoblastic cell line, UMR-106. The present study shows that UMR-106 expresses the AhR and that the expression of CYP1A1 is induced after exposure to TCDD, while down-regulation of OPN is an even more rapid response and a sensitive biomarker to TCDD exposure in this osteoblastic cell line. In conclusion, this osteoblastic cell line may be used as an in vitro model-system for studying dioxin-induced effects on osteoblasts.

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