2. Academic Validation
  3. The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment

The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment

  • Antimicrob Agents Chemother. 2006 Apr;50(4):1522-4. doi: 10.1128/AAC.50.4.1522-1524.2006.
Jon-Paul S Powers 1,  Morgan M Martin,  Danika L Goosney,  Robert E W Hancock
Affiliations

Affiliation

  • 1 Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
PMID: 16569873 DOI: 10.1128/AAC.50.4.1522-1524.2006
Abstract

The horseshoe crab peptide polyphemusin I possesses high antimicrobial activity, but its mechanism of action is as yet not well defined. Using a biotin-labeled polyphemusin I analogue and confocal fluorescence microscopy, we showed that the peptide accumulates in the cytoplasm of wild-type Escherichia coli within 30 min after addition without causing substantial membrane damage.

Figures
Products