2. Academic Validation
  3. Members of adenovirus species B utilize CD80 and CD86 as cellular attachment receptors

Members of adenovirus species B utilize CD80 and CD86 as cellular attachment receptors

  • Virus Res. 2006 Dec;122(1-2):144-53. doi: 10.1016/j.virusres.2006.07.009.
Joshua J Short 1,  Chenthamarakshan Vasu,  Mark J Holterman,  David T Curiel,  Alexander Pereboev
Affiliations

Affiliation

  • 1 Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, and Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL 35294-2172, USA.
PMID: 16920215 DOI: 10.1016/j.virusres.2006.07.009
Abstract

Alternate serotypes of adenovirus (Ad), including Ads of species B, are being explored to circumvent the disadvantages of Ad serotype 5 gene delivery vectors. Whereas the majority of human Ads utilize the Coxsackievirus and adenovirus receptor (CAR), none of the Ad species B use CAR. Ad species B is further divided into two subspecies, B1 and B2, and utilizes at least two classes of receptors: common Ad species B receptors and B2 specific receptors. CD46 has been implicated as a B2-specific receptor. Ad serotype 3 (Ad3), a member of B1, utilizes CD80 and CD86 as cellular attachment receptors. The receptor-interacting Ad fiber-knob domain is highly homologous among species B Ads. We hypothesized that Other members of Ad species B may utilize CD80 and CD86 as cellular attachment receptors. All tested species B members showed specific binding to cells expressing CD80 and CD86, and the Ad fiber-knob domain from both B1 and B2 Ad efficiently blocked CD80- and CD86-mediated Infection of Ad3 vectors. Members of both B1 and B2 demonstrated CD80- and CD86-specific Infection of CHO cells expressing CD80 and CD86. Therefore, all of the members of Ad species B utilize CD80 and CD86 for Infection of cells.

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