2. Academic Validation
  3. Bongkrekic acid and atractyloside inhibits chloride channels from mitochondrial membranes of rat heart

Bongkrekic acid and atractyloside inhibits chloride channels from mitochondrial membranes of rat heart

  • Biochim Biophys Acta. 2007 Jan;1767(1):31-44. doi: 10.1016/j.bbabio.2006.10.004.
Lubica Malekova 1,  Viera Kominkova,  Miroslav Ferko,  Peter Stefanik,  Olga Krizanova,  Attila Ziegelhöffer,  Adam Szewczyk,  Karol Ondrias
Affiliations

Affiliation

  • 1 Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34 Bratislava, Slovak Republic.
PMID: 17123460 DOI: 10.1016/j.bbabio.2006.10.004
Abstract

The aim of this work was to characterize the effect of bongkrekic acid (BKA), atractyloside (ATR) and carboxyatractyloside (CAT) on single channel properties of chloride channels from mitochondria. Mitochondrial membranes isolated from a rat heart muscle were incorporated into a bilayer lipid membrane (BLM) and single Chloride Channel currents were measured in 250/50 mM KCl cis/trans solutions. BKA (1-100 microM), ATR and CAT (5-100 microM) inhibited the chloride channels in dose-dependent manner. The inhibitory effect of the BKA, ATR and CAT was pronounced from the trans side of a BLM and it increased with time and at negative voltages (trans-cis). These compounds did not influence the single channel amplitude, but decreased open dwell time of channels. The inhibitory effect of BKA, ATR and CAT on the mitochondrial Chloride Channel may help to explain some of their cellular and/or subcellular effects.

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