2. Academic Validation
  3. Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1

Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1

  • Dev Cell. 2007 Jan;12(1):17-30. doi: 10.1016/j.devcel.2006.11.002.
Yohta Fujita 1,  Takeshi Hayashi,  Tomomi Kiyomitsu,  Yusuke Toyoda,  Aya Kokubu,  Chikashi Obuse,  Mitsuhiro Yanagida
Affiliations

Affiliation

  • 1 CREST Research Project of Japan Science and Technology Corporation (JST), Graduate School of Biostudies, Kyoto University, Yoshida-Honmachi, Kyoto 606-8501, Japan.
PMID: 17199038 DOI: 10.1016/j.devcel.2006.11.002
Abstract

The centromere is the chromosomal site that joins to microtubules during mitosis for proper segregation. Determining the location of a centromere-specific histone H3 called CENP-A at the centromere is vital for understanding centromere structure and function. Here, we report the identification of three human proteins essential for centromere/kinetochore structure and function, hMis18alpha, hMis18beta, and M18BP1, the complex of which is accumulated specifically at the telophase-G1 centromere. We provide evidence that such centromeric localization of hMis18 is essential for the subsequent recruitment of de novo-synthesized CENP-A. If any of the three is knocked down by RNAi, centromere recruitment of newly synthesized CENP-A is rapidly abolished, followed by defects such as misaligned chromosomes, anaphase missegregation, and interphase micronuclei. Tricostatin A, an inhibitor to histone deacetylase, suppresses the loss of CENP-A recruitment to centromeres in hMis18alpha RNAi cells. Telophase centromere chromatin may be primed or licensed by the hMis18 complex and RbAp46/48 to recruit CENP-A through regulating the acetylation status in the centromere.

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