1. Academic Validation
  2. PLU-1 is an H3K4 demethylase involved in transcriptional repression and breast cancer cell proliferation

PLU-1 is an H3K4 demethylase involved in transcriptional repression and breast cancer cell proliferation

  • Mol Cell. 2007 Mar 23;25(6):801-12. doi: 10.1016/j.molcel.2007.03.001.
Kenichi Yamane 1 Keisuke Tateishi Robert J Klose Jia Fang Laura A Fabrizio Hediye Erdjument-Bromage Joyce Taylor-Papadimitriou Paul Tempst Yi Zhang
Affiliations

Affiliation

  • 1 Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA.
Abstract

Posttranslational modification of chromatin by histone methylation has wide-ranging effects on nuclear function, including transcriptional regulation, maintenance of genome integrity, and epigenetic inheritance. The Enzymes utilized to place histone methylation marks are well characterized, but the identity of a histone demethylation system remained elusive until recently. The discovery of Histone Demethylase enzymes capable of directly removing methyl groups from modified lysine residues has demonstrated that histone methylation is a dynamic modification. The most extensive family of Histone Demethylase enzymes identified so far contains a JmjC domain and catalyzes demethylation through a hydroxylation reaction. Here, we identify PLU-1, a transcriptional repressor implicated in breast Cancer, as a Histone Demethylase enzyme that has the ability to reverse the trimethyl H3K4 modification state. Furthermore, we reveal that PLU-1-mediated H3K4 demethylase activity plays an important role in the proliferative capacity of breast Cancer cells through repression of tumor suppressor genes, including BRCA1.

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