The S2 subsites of cathepsins K and L and their contribution to collagen degradation

The exchange of residues 67 and 205 of the S2 pocket of human cysteine cathepsins K and L induces a permutation of their substrate specificity toward fluorogenic peptide substrates. While the Cathepsin L-like Cathepsin K (Tyr67Leu/Leu205Ala) mutant has a marked preference for Phe, the Leu67Tyr/Ala205Leu Cathepsin L variant shows an effective Cathepsin K-like preference for Leu and Pro. A similar turnaround of inhibition was observed by using specific inhibitors of Cathepsin K [1-(N-Benzyloxycarbonyl-leucyl)-5-(N-Boc-phenylalanyl-leucyl)carbohydrazide] and Cathepsin L [N-(4-biphenylacetyl)-S-methylcysteine-(D)-Arg-Phe-beta-phenethylamide]. Molecular modeling studies indicated that mutations alter the character of both S2 and S3 subsites, while docking calculations were consistent with kinetics data. The Cathepsin K-like Cathepsin L was unable to mimic the collagen-degrading activity of Cathepsin K against collagens I and II, DQ-collagens I and IV, and elastin-Congo Red. In summary, double mutations of the S2 pocket of cathepsins K (Y67L/L205A) and L (L67Y/A205L) induce a switch of their enzymatic specificity toward small selective inhibitors and peptidyl substrates, confirming the key role of residues 67 and 205. However, mutations in the S2 subsite pocket of Cathepsin L alone without engineering of binding sites to chondroitin sulfate are not sufficient to generate a Cathepsin K-like collagenase, emphasizing the pivotal role of the complex formation between glycosaminoglycans and Cathepsin K for its unique collagenolytic activity.