2. Academic Validation
  3. CFTR stabilizes ENaC at the plasma membrane

CFTR stabilizes ENaC at the plasma membrane

  • J Cyst Fibros. 2007 Nov 30;6(6):419-22. doi: 10.1016/j.jcf.2007.03.001.
C Lu 1,  C Jiang,  S Pribanic,  D Rotin
Affiliations

Affiliation

  • 1 Program in Cell Biology, The Hospital for Sick Children, and Biochemistry Department, University of Toronto, 555 University Ave, Toronto, Ontario, Canada M5G 1X8.
PMID: 17434346 DOI: 10.1016/j.jcf.2007.03.001
Abstract

CFTR was reported to regulate ENaC channel opening, decreasing ENaC activity in airways and increasing it in sweat ducts. We generated MDCK-I cell lines stably expressing tagged alphabetagammaENaC+CFTR or ENaC alone, and developed an assay to quantify cell-surface half-life of ENaC. Surprisingly, we found that co-expressed CFTR stabilizes ENaC at the plasma membrane, suggesting that CFTR regulates ENaC stability, not just opening.

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