2. Academic Validation
  3. Isolation of coniferyl esters from Capsicum baccatum L., and their enzymatic preparation and agonist activity for TRPV1

Isolation of coniferyl esters from Capsicum baccatum L., and their enzymatic preparation and agonist activity for TRPV1

  • Phytochemistry. 2008 Mar;69(5):1179-84. doi: 10.1016/j.phytochem.2007.11.017.
Kenji Kobata 1,  Hitomi Tate,  Yusaku Iwasaki,  Yoshiyuki Tanaka,  Keigo Ohtsu,  Susumu Yazawa,  Tatsuo Watanabe
Affiliations

Affiliation

  • 1 School of Food and Nutritional Sciences and COE program in the 21st century, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
PMID: 18190936 DOI: 10.1016/j.phytochem.2007.11.017
Abstract

Coniferyl esters--capsiconiate and dihydrocapsiconiate--were isolated from the fruits of the pepper, Capsicum baccatum L. var. praetermissum. Their structures were determined by spectroscopic methods to be coniferyl (E)-8-methyl-6-nonenoate (capsiconiate) and coniferyl 8-methylnonanoate (dihydrocapsiconiate). This finding was further confirmed by the lipase-catalyzed condensation of coniferyl alcohol with its corresponding fatty acid derivative. The agonist activity of the esters for transient receptor potential vanilloid 1 (TRPV1) was evaluated by conducting an analysis of the intracellular calcium concentrations in TRPV1-expressing HEK293 cells. The EC50 values of capsiconiate and dihydrocapsiconiate were 3.2 and 4.2 microM, respectively.

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