2. Academic Validation
  3. The ARID domain of the H3K4 demethylase RBP2 binds to a DNA CCGCCC motif

The ARID domain of the H3K4 demethylase RBP2 binds to a DNA CCGCCC motif

  • Nat Struct Mol Biol. 2008 Apr;15(4):419-21. doi: 10.1038/nsmb.1400.
Shengjiang Tu 1,  Yu-Ching Teng,  Chunhua Yuan,  Ying-Ta Wu,  Meng-Yu Chan,  An-Ning Cheng,  Po-Hsun Lin,  Li-Jung Juan,  Ming-Daw Tsai
Affiliations

Affiliation

  • 1 Department of Chemistry, Ohio State University, 100 West 18th Ave., Columbus, Ohio 43210, USA.
PMID: 18270511 DOI: 10.1038/nsmb.1400
Abstract

The histone H3 lysine 4 demethylase RBP2 contains a DNA binding domain, the AT-rich interaction domain (ARID). We solved the structure of ARID by NMR, identified its DNA binding motif (CCGCCC) and characterized the binding contacts. Immunofluorescence and luciferase assays indicated that ARID is required for RBP2 demethylase activity in cells and that DNA recognition is essential to regulate transcription.

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