2. Academic Validation
  3. Cloning of a human gene encoding the general transcription initiation factor IIB

Cloning of a human gene encoding the general transcription initiation factor IIB

  • Nature. 1991 Aug 22;352(6337):689-95. doi: 10.1038/352689a0.
I Ha 1,  W S Lane,  D Reinberg
Affiliations

Affiliation

  • 1 Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
PMID: 1876184 DOI: 10.1038/352689a0
Abstract

Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic sigma-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.

Figures