1. Academic Validation
  2. Viewing membrane-bound molecular umbrellas by parallax analyses

Viewing membrane-bound molecular umbrellas by parallax analyses

  • J Am Chem Soc. 2008 Oct 15;130(41):13771-7. doi: 10.1021/ja804929m.
Masaharu Kondo 1 Mohamed Mehiri Steven L Regen
Affiliations

Affiliation

  • 1 Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
Abstract

Fluorescence quenching measurements have been made for a series of di-walled and tetra-walled molecular umbrellas having moderate (i.e., hydroxyl-) and strong (i.e., sulfate-) facial hydrophilicity, using Cascade Blue as the fluorophore. Through the use of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphotempocholine, 1-palmitoyl-2-stearoyl-(5-DOXYL)-sn-glycero-3-phosphocholine, and 1-palmitoyl-2-stearoyl-(12-DOXYL)-sn-glycero-3-phosphocholine as fluorescence quenchers, evidence has been obtained for a membrane-bound state in which the umbrella molecules lie on the surface of the lipid bilayer. In the case of the sulfated molecular umbrellas, evidence has also been obtained for a subpopulation in which the fluorophore lies deeper within the membrane. Probable structures for the shallow-lying and deep-lying molecular umbrellas are discussed.

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