2. Academic Validation
  3. Roxithromycin inhibits chemokine-induced chemotaxis of Th1 and Th2 cells but regulatory T cells

Roxithromycin inhibits chemokine-induced chemotaxis of Th1 and Th2 cells but regulatory T cells

  • J Dermatol Sci. 2009 Jun;54(3):185-91. doi: 10.1016/j.jdermsci.2009.01.007.
Taisuke Ito 1,  Natsuho Ito,  Hideo Hashizume,  Masahiro Takigawa
Affiliations

Affiliation

  • 1 Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan. itoutai@hama-med.ac.jp
PMID: 19251402 DOI: 10.1016/j.jdermsci.2009.01.007
Abstract

Background: Roxithromycin (RXM), a 14-member macrolide Antibiotic, has a variety of bioregulatory functions such as anti-inflammatory effects, anti-oxidant effects, and modulation of immune responses.

Objectives: In this study, we analyzed the effect of RXM on chemokine-induced chemotaxis of Th1, Th2, and regulatory T (Treg) cells established from three normal human peripheral blood lymphocytes by the reported methods.

Methods and results: Incubation with 10 microM RXM for 18 h did not alter the expression profile of CXCR3 on Th1 cells and CCR4 on Th2 and Treg cells. However, upon RXM preincubation, the migration of Th1 cells to IP-10 and Th2 cells to TARC was partially suppressed, although RXM did not influence Treg cell migration. Erythromycin and clarithromycin at the same concentration did not exert such effects. F-actin polymerization and CA(++) influx induced by IP-10 and TARC in Th1 and Th2 cells, respectively, was down-regulated by RXM pretreatment.

Conclusion: These results imply that RXM exhibits bioregulatory function by influencing chemotaxis of Th1 and Th2 cells while leaving Treg cell migration unaffected.

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