Roxithromycin (Synonyms: RU-28965)

Name: Roxithromycin
Brand: MedChemExpress
SKU: HY-B0435
Rating: 5.0 (4 reviews)

Cat. No.: HY-B0435 Purity: 99.93%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Roxithromycin (RU-28965) is a semi-synthetic macrolide antibiotic.

For research use only. We do not sell to patients.

Roxithromycin Chemical Structure

CAS No. : 80214-83-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 43	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 43	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	USD 39	In-stock	Estimated Time of Arrival: December 31
5 g	USD 86	In-stock	Estimated Time of Arrival: December 31
10 g	USD 130	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Roxithromycin:

Roxithromycin-d₇ Get quote

4 Publications Citing Use of MCE Roxithromycin

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Roxithromycin (RU-28965) is a semi-synthetic macrolide antibiotic.

IC₅₀ & Target

Macrolide

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	160 μg/mL Compound: Roxithromycin	Antiproliferative effect against HeLa cells after 48 hrs Antiproliferative effect against HeLa cells after 48 hrs	[PMID: 17088489]
HeLa	IC₅₀	90 μg/mL Compound: Roxithromycin	Inhibition of metabolic activity in HeLa cells assessed as MTT reduction after 48 hrs Inhibition of metabolic activity in HeLa cells assessed as MTT reduction after 48 hrs	[PMID: 17088489]
HUVEC	IC₅₀	100 μM Compound: RXM	Inhibition of cell proliferation of HUVEC at 12.5 to 200 uM after 72 hrs by MTS assay Inhibition of cell proliferation of HUVEC at 12.5 to 200 uM after 72 hrs by MTS assay	[PMID: 25528335]
MG-63	IC₅₀	110 μg/mL Compound: Roxithromycin	Inhibition of metabolic activity in MG63 cells assessed as MTT reduction after 48 hrs Inhibition of metabolic activity in MG63 cells assessed as MTT reduction after 48 hrs	[PMID: 17088489]
MG-63	IC₅₀	180 μg/mL Compound: Roxithromycin	Antiproliferative effect against MG63 cells assessed as BrdU incorporation into DNA after 48 hrs after 48 hrs Antiproliferative effect against MG63 cells assessed as BrdU incorporation into DNA after 48 hrs after 48 hrs	[PMID: 17088489]
Osteoblast	IC₅₀	210 μg/mL Compound: Roxithromycin	Inhibition of metabolic activity in primary human osteoblasts assessed as MTT reduction after 48 hrs Inhibition of metabolic activity in primary human osteoblasts assessed as MTT reduction after 48 hrs	[PMID: 17088489]
Osteoblast	IC₅₀	70 μg/mL Compound: Roxithromycin	Antiproliferative effect against primary human osteoblasts assessed as BrdU incorporation into DNA after 48 hrs Antiproliferative effect against primary human osteoblasts assessed as BrdU incorporation into DNA after 48 hrs	[PMID: 17088489]

Clinical Trial

Molecular Weight

837.05

Formula

C₄₁H₇₆N₂O₁₅

CAS No.

80214-83-1

Appearance

Solid

Color

White to off-white

SMILES

C[C@H]([C@H]([C@](O)(C[C@H](/C1=N\OCOCCOC)C)C)O[C@@](O[C@H](C)C[C@@H]2N(C)C)([H])[C@@H]2O)[C@]([C@H](C(O[C@@H]([C@@](O)([C@H](O)[C@H]1C)C)CC)=O)C)([H])O[C@@](O[C@@H](C)[C@@H]3O)([H])C[C@@]3(C)OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (119.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1947 mL	5.9734 mL	11.9467 mL
5 mM	0.2389 mL	1.1947 mL	2.3893 mL
10 mM	0.1195 mL	0.5973 mL	1.1947 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (2.99 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (273 KB) SDS (398 KB)

COA (252 KB) HNMR (337 KB) RP-HPLC (190 KB)

Handling Instructions (2659 KB)

References

[1]. http://en.wikipedia.org/wiki/Roxithromycin

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1947 mL	5.9734 mL	11.9467 mL	29.8668 mL
	5 mM	0.2389 mL	1.1947 mL	2.3893 mL	5.9734 mL
	10 mM	0.1195 mL	0.5973 mL	1.1947 mL	2.9867 mL
	15 mM	0.0796 mL	0.3982 mL	0.7964 mL	1.9911 mL
	20 mM	0.0597 mL	0.2987 mL	0.5973 mL	1.4933 mL
	25 mM	0.0478 mL	0.2389 mL	0.4779 mL	1.1947 mL
	30 mM	0.0398 mL	0.1991 mL	0.3982 mL	0.9956 mL
	40 mM	0.0299 mL	0.1493 mL	0.2987 mL	0.7467 mL
	50 mM	0.0239 mL	0.1195 mL	0.2389 mL	0.5973 mL
	60 mM	0.0199 mL	0.0996 mL	0.1991 mL	0.4978 mL
	80 mM	0.0149 mL	0.0747 mL	0.1493 mL	0.3733 mL
	100 mM	0.0119 mL	0.0597 mL	0.1195 mL	0.2987 mL

Roxithromycin Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Roxithromycin80214-83-1RU-28965RU28965RU 28965BacterialAntibioticInhibitorinhibitorinhibit

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Roxithromycin (Synonyms: RU-28965)

Customer Review

Other Forms of Roxithromycin:

Roxithromycin Related Antibodies

4 Publications Citing Use of MCE Roxithromycin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Roxithromycin Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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