2. Academic Validation
  3. Conjugation with alpha-linolenic acid improves cancer cell uptake and cytotoxicity of doxorubicin

Conjugation with alpha-linolenic acid improves cancer cell uptake and cytotoxicity of doxorubicin

  • Bioorg Med Chem Lett. 2009 May 1;19(9):2579-84. doi: 10.1016/j.bmcl.2009.03.016.
Meng-Lei Huan 1,  Si-Yuan Zhou,  Zeng-Hui Teng,  Bang-le Zhang,  Xin-You Liu,  Jie-Pin Wang,  Qi-Bing Mei
Affiliations

Affiliation

  • 1 Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, 17, Changle West Road, Xi'an, Shaan'xi 710032, China.
PMID: 19329312 DOI: 10.1016/j.bmcl.2009.03.016
Abstract

The synthetic DOX-LNA conjugate was characterized by proton nuclear magnetic resonance and mass spectrometry. In addition, the purity of the conjugate was analyzed by reverse-phase high-performance liquid chromatography. The cellular uptake, intracellular distribution, and cytotoxicity of DOX-LNA were assessed by flow cytometry, fluorescence microscopy, liquid chromatography/electrospray ionization tandem mass spectrometry, and the tetrazolium dye assay using the in vitro cell models. The DOX-LNA conjugate showed substantially higher tumor-specific cytotoxicity compared with DOX.

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