2. Academic Validation
  3. A regulated RNA binding protein also possesses aconitase activity

A regulated RNA binding protein also possesses aconitase activity

  • Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10109-13. doi: 10.1073/pnas.88.22.10109.
S Kaptain 1,  W E Downey,  C Tang,  C Philpott,  D Haile,  D G Orloff,  J B Harford,  T A Rouault,  R D Klausner
Affiliations

Affiliation

  • 1 Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
PMID: 1946430 DOI: 10.1073/pnas.88.22.10109
Abstract

A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of mitochondrial aconitase are identical to those in the IRE-BP, suggesting that the IRE-BP may possess aconitase activity. After purification of native IRE-BP and immunoaffinity purification of transfected and expressed IRE-BP, we demonstrate that the purified IRE-BP has aconitase activity.

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