2. Academic Validation
  3. Structure-activity relationships of monomeric C2-aryl pyrrolo[2,1-c][1,4]benzodiazepine (PBD) antitumor agents

Structure-activity relationships of monomeric C2-aryl pyrrolo[2,1-c][1,4]benzodiazepine (PBD) antitumor agents

  • J Med Chem. 2010 Apr 8;53(7):2927-41. doi: 10.1021/jm901722v.
Dyeison Antonow 1,  Maciej Kaliszczak,  Gyoung-Dong Kang,  Marissa Coffils,  Arnaud C Tiberghien,  Nectaroula Cooper,  Teresa Barata,  Sibylle Heidelberger,  Colin H James,  Mire Zloh,  Terence C Jenkins,  Anthony P Reszka,  Stephen Neidle,  Sylvie M Guichard,  Duncan I Jodrell,  John A Hartley,  Philip W Howard,  David E Thurston
Affiliations

Affiliation

  • 1 Cancer Research UK Gene Targeted Drug Design Research Group, The School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, U.K.
PMID: 20218628 DOI: 10.1021/jm901722v
Abstract

A comprehensive SAR investigation of the C2-position of pyrrolo[2,1-c][1,4]benzodiazepine (PBD) monomer antitumor agents is reported, establishing the molecular requirements for optimal in vitro cytotoxicity and DNA-binding affinity. Both carbocyclic and heterocyclic C2-aryl substituents have been studied ranging from single aryl rings to fused ring systems, and also styryl substituents, establishing across a library of 80 analogues that C2-aryl and styryl substituents significantly enhance both DNA-binding affinity and in vitro cytotoxicity, with a correlation between the two. The optimal C2-grouping for both DNA-binding affinity and cytotoxicity was found to be the C2-quinolinyl moiety which, according to molecular modeling, is due to the overall fit of the molecule in the DNA minor groove, and potential specific contacts with functional groups in the floor and walls of the groove. This analogue (14l) was shown to delay tumor growth in a HCT-116 (bowel) human tumor xenograft model.

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