2. Academic Validation
  3. Sorting of the Alzheimer's disease amyloid precursor protein mediated by the AP-4 complex

Sorting of the Alzheimer's disease amyloid precursor protein mediated by the AP-4 complex

  • Dev Cell. 2010 Mar 16;18(3):425-36. doi: 10.1016/j.devcel.2010.01.015.
Patricia V Burgos 1,  Gonzalo A Mardones,  Adriana L Rojas,  Luis L P daSilva,  Yogikala Prabhu,  James H Hurley,  Juan S Bonifacino
Affiliations

Affiliation

  • 1 Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
PMID: 20230749 DOI: 10.1016/j.devcel.2010.01.015
Abstract

Adaptor protein 4 (AP-4) is the most recently discovered and least well-characterized member of the family of heterotetrameric adaptor protein (AP) complexes that mediate sorting of transmembrane cargo in post-Golgi compartments. Herein, we report the interaction of an YKFFE sequence from the cytosolic tail of the Alzheimer's disease amyloid precursor protein (APP) with the mu4 subunit of AP-4. Biochemical and X-ray crystallographic analyses reveal that the properties of the APP sequence and the location of the binding site on mu4 are distinct from those of Other signal-adaptor interactions. Disruption of the APP-AP-4 interaction decreases localization of APP to endosomes and enhances gamma-secretase-catalyzed cleavage of APP to the pathogenic amyloid-beta peptide. These findings demonstrate that APP and AP-4 engage in a distinct type of signal-adaptor interaction that mediates transport of APP from the trans-Golgi network (TGN) to endosomes, thereby reducing amyloidogenic processing of the protein.

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