1. Academic Validation
  2. Protoflavonoids from ferns impair centrosomal integrity of tumor cells

Protoflavonoids from ferns impair centrosomal integrity of tumor cells

  • Planta Med. 2011 Mar;77(5):461-6. doi: 10.1055/s-0030-1250407.
Isabelle Pouny 1 Chantal Etiévant Laurence Marcourt Isabelle Huc-Dumas Muriel Batut Frédéric Girard Michel Wright Georges Massiot
Affiliations

Affiliation

  • 1 Unité Mixte de Service 2597, Centre National de la Recherche Scientifique (CNRS)-Pierre Fabre, Institut des Sciences et Technologies du Médicament de Toulouse (ISTMT), Toulouse, France.
Abstract

Six protoflavonoids, including two new compounds, were isolated during a large scale screening of fern extracts for original interaction with mitosis. The new compounds isolated from PHEGOPTERIS decursive-pinnata and EQUISETUM fluviatile were 2',3'-dihydroprotogenkwanone (1) and 2',3'-dihydro-2'-hydroxyprotoapigenone (2). Known compounds were: protoapigenone, protogenkwanone, protoapigenin, and 4'- O- β-D-glucopyranosyl protoapigenin. They showed a cytotoxic activity against HeLa cells at a micromolar level. IC₅₀ values were 2 µM for compound 1 > 10 µM for compound 2, and respectively 2.4, 0.6, > 10 µM for the known compounds. Their cytotoxic effects were associated with phenotypic changes never observed before and characterized by the loss of centrosomal γ-tubulin labelling in both mitotic and interphasic cells.

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