Protein tyrosine kinase 7 has a conserved role in Wnt/β-catenin canonical signalling

EMBO Rep. 2011 Jan;12(1):43-9. doi: 10.1038/embor.2010.185.

Francesca Puppo ¹, Virginie Thomé, Anne-Catherine Lhoumeau, Marie Cibois, Akanksha Gangar, Frédérique Lembo, Edwige Belotti, Sylvie Marchetto, Patrick Lécine, Thomas Prébet, Michael Sebbagh, Won-Sik Shin, Seung-Taek Lee, Laurent Kodjabachian, Jean-Paul Borg

Affiliations

Affiliation

1 Inserm, U891, Centre de Recherche en Cancérologie de Marseille, 27 Boulevard Lei Roure, Marseille F-13009.

PMID: 21132015 DOI: 10.1038/embor.2010.185

Abstract

The receptor protein tyrosine kinase 7 (PTK7) was recently shown to participate in noncanonical Wnt/planar cell polarity signalling during mouse and frog embryonic development. In this study, we report that PTK7 interacts with β-catenin in a yeast two-hybrid assay and mammalian cells. PTK7-deficient cells exhibit weakened β-catenin/T-cell factor transcriptional activity on Wnt3a stimulation. Furthermore, Xenopus PTK7 is required for the formation of Spemann's organizer and for Siamois promoter activation, events that require β-catenin transcriptional activity. Using epistatic assays, we demonstrate that PTK7 functions upstream from glycogen synthase kinase 3. Taken together, our data reveal a new and conserved role for PTK7 in the Wnt canonical signalling pathway.

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