2. Academic Validation
  3. The structure of human 5-lipoxygenase

The structure of human 5-lipoxygenase

  • Science. 2011 Jan 14;331(6014):217-9. doi: 10.1126/science.1197203.
Nathaniel C Gilbert 1,  Sue G Bartlett,  Maria T Waight,  David B Neau,  William E Boeglin,  Alan R Brash,  Marcia E Newcomer
Affiliations

Affiliation

  • 1 Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
PMID: 21233389 DOI: 10.1126/science.1197203
Abstract

The synthesis of both proinflammatory leukotrienes and anti-inflammatory lipoxins requires the Enzyme 5-lipoxygenase (5-LOX). 5-LOX activity is short-lived, apparently in part because of an intrinsic instability of the Enzyme. We identified a 5-LOX-specific destabilizing sequence that is involved in orienting the carboxyl terminus, which binds the catalytic iron. Here, we report the crystal structure at 2.4 angstrom resolution of human 5-LOX stabilized by replacement of this sequence.

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