2. Academic Validation
  3. Next-generation sequencing to generate interactome datasets

Next-generation sequencing to generate interactome datasets

  • Nat Methods. 2011 Jun;8(6):478-80. doi: 10.1038/nmeth.1597.
Haiyuan Yu 1,  Leah Tardivo,  Stanley Tam,  Evan Weiner,  Fana Gebreab,  Changyu Fan,  Nenad Svrzikapa,  Tomoko Hirozane-Kishikawa,  Edward Rietman,  Xinping Yang,  Julie Sahalie,  Kourosh Salehi-Ashtiani,  Tong Hao,  Michael E Cusick,  David E Hill,  Frederick P Roth,  Pascal Braun,  Marc Vidal
Affiliations

Affiliation

  • 1 Center for Cancer Systems Biology, Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
PMID: 21516116 DOI: 10.1038/nmeth.1597
Abstract

Next-generation Sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse Sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation Sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.

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