2. Academic Validation
  3. D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine

D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine

  • Nat Chem Biol. 2011 Apr 27;7(8):528-30. doi: 10.1038/nchembio.586.
Susan E Cellitti 1,  Weijia Ou,  Hsien-Po Chiu,  Jan Grünewald,  David H Jones,  Xueshi Hao,  Qian Fan,  Lisa L Quinn,  Kenneth Ng,  Andrew T Anfora,  Scott A Lesley,  Tetsuo Uno,  Ansgar Brock,  Bernhard H Geierstanger
Affiliations

Affiliation

  • 1 Genomics Institute of the Novartis Research Foundation, San Diego, California, USA.
PMID: 21525873 DOI: 10.1038/nchembio.586
Abstract

D-ornithine has previously been suggested to enhance the expression of pyrrolysine-containing proteins. We unexpectedly discovered that uptake of D-ornithine results in the insertion of a new amino acid, pyrroline-carboxy-lysine (Pcl) instead of the anticipated pyrrolysine (Pyl). Our feeding and biochemical studies point to specific roles of the poorly understood Pyl biosynthetic Enzymes PylC and PylD in converting L-lysine and D-ornithine to Pcl and confirm intermediates in the biosynthesis of Pyl.

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