2. Academic Validation
  3. A universal code for RNA recognition by PUF proteins

A universal code for RNA recognition by PUF proteins

  • Nat Chem Biol. 2011 May 15;7(7):425-7. doi: 10.1038/nchembio.577.
Aleksandra Filipovska 1,  Muhammad F M Razif,  Karoline K A Nygård,  Oliver Rackham
Affiliations

Affiliation

  • 1 Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Perth, Australia.
PMID: 21572425 DOI: 10.1038/nchembio.577
Abstract

The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of diverse sequence and structure.

Figures