2. Academic Validation
  3. Novel cyanine dyes with vinylsulfone group for labeling biomolecules

Novel cyanine dyes with vinylsulfone group for labeling biomolecules

  • Bioconjug Chem. 2012 Mar 21;23(3):350-62. doi: 10.1021/bc200232d.
Jin Woo Park 1,  YoungSoo Kim,  Kee-Jung Lee,  Dong Jin Kim
Affiliations

Affiliation

  • 1 Center for Neuro-Medicine, Brain Science Institute, Korea Institute of Science and Technology, L2317, 39-1 Hawolgok-dong, Sungbuk-ku, Seoul, Korea 136-791.
PMID: 22243689 DOI: 10.1021/bc200232d
Abstract

Novel vinylsulfone cyanine dyes (em. 550-850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.

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