2. Academic Validation
  3. Catalyst-free and site-specific one-pot dual-labeling of a protein directed by two genetically incorporated noncanonical amino acids

Catalyst-free and site-specific one-pot dual-labeling of a protein directed by two genetically incorporated noncanonical amino acids

  • Chembiochem. 2012 Jul 9;13(10):1405-8. doi: 10.1002/cbic.201200281.
Bo Wu 1,  Zhiyong Wang,  Ying Huang,  Wenshe R Liu
Affiliations

Affiliation

  • 1 Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
PMID: 22628069 DOI: 10.1002/cbic.201200281
Abstract

The genetic incorporation of one azide-containing and one keto-containing noncanonical Amino acids into a protein at one amber and one ochre mutation sites respectively followed by their orthogonal reactions with one hydroxylamine-containing and one cyclooctyne-containing dyes allows highly efficient one-pot site-specific dual labeling of the protein in a catalyst-free fashion.

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