2. Academic Validation
  3. MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins

MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins

  • FEBS Lett. 2012 Oct 19;586(20):3555-61. doi: 10.1016/j.febslet.2012.07.043.
Stefan M K Davies 1,  Maria I G Lopez Sanchez,  Reena Narsai,  Anne-Marie J Shearwood,  Muhammad F M Razif,  Ian D Small,  James Whelan,  Oliver Rackham,  Aleksandra Filipovska
Affiliations

Affiliation

  • 1 Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Perth, Western Australia 6000, Australia.
PMID: 22841715 DOI: 10.1016/j.febslet.2012.07.043
Abstract

Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNA(Glu), however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity.

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