Effects of kisspeptin-13 on the hypothalamic-pituitary-adrenal axis, thermoregulation, anxiety and locomotor activity in rats

Kisspeptin is a mammalian amidated neurohormone, which belongs to the RF-amide peptide family and is known for its key role in reproduction. However, in contrast with the related members of the RF-amide family, little information is available regarding its role in the stress-response. With regard to the recent data suggesting kisspeptin neuronal projections to the paraventricular nucleus, in the present experiments we investigated the effect of kisspeptin-13 (KP-13), an endogenous derivative of kisspeptin, on the hypothalamus-pituitary-adrenal (HPA) axis, motor behavior and thermoregulatory function. The peptide was administered intracerebroventricularly (icv.) in different doses (0.5-2 μg) to adult male Sprague-Dawley rats, the behavior of which was then observed by means of telemetry, open field and elevated plus maze tests. Additionally, plasma concentrations of corticosterone were measured in order to assess the influence of KP-13 on the HPA system. The effects on core temperature were monitored continuously via telemetry. The results demonstrated that KP-13 stimulated the horizontal locomotion (square crossing) in the open field test and decreased the number of entries into and the time spent in the open arms during the elevated plus maze tests. The peptide also caused marked elevations in the spontaneous locomotor activity and the core temperature recorded by the telemetric system, and significantly increased the basal corticosterone level. In conclusion, our data indicate that icv. administered KP-13 stimulates the HPA axis, induces hyperthermia, activates motor behavior and causes anxiety in rats.