2. Academic Validation
  3. SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal

SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal

  • Epigenetics. 2013 Feb;8(2):177-83. doi: 10.4161/epi.23416.
Olivier Binda 1,  Ana Sevilla,  Gary LeRoy,  Ihor R Lemischka,  Benjamin A Garcia,  Stéphane Richard
Affiliations

Affiliation

  • 1 Terry Fox Molecular Oncology Group, Bloomfield Center for Research on Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Department of Oncology, McGill University, Montréal, QC, Canada.
PMID: 23324626 DOI: 10.4161/epi.23416
Abstract

The histone H2A variant H2AZ is an essential chromatin signaling factor. Herein, we report that H2AZ is monomethylated at lysine 7 (H2AZK7me1) by the lysine methyltransferase SETD6. We observed that methylation of H2AZ increased noticeably upon cellular differentiation of mouse embryonic stem cells (mESCs). H2AZK7me1 and the repressive H3K27me3 MARK were found near the transcriptional start sites of differentiation marker genes, but were removed upon retinoic acid-induced cellular differentiation. The depletion of Setd6 in mESCs led to cellular differentiation, compromised self-renewal, and poor clonogenicity. These findings demonstrate that mESCs require Setd6 for self-renewal and portray H2AZK7me1 as a marker of cellular differentiation.

Keywords

H2AZ; SETD6; embryonic stem cells; histone variant; lysine methylation; lysine methyltransferase.

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