2. Academic Validation
  3. A Y2H-seq approach defines the human protein methyltransferase interactome

A Y2H-seq approach defines the human protein methyltransferase interactome

  • Nat Methods. 2013 Apr;10(4):339-42. doi: 10.1038/nmeth.2397.
Mareike Weimann 1,  Arndt Grossmann,  Jonathan Woodsmith,  Ziya Özkan,  Petra Birth,  David Meierhofer,  Nouhad Benlasfer,  Taras Valovka,  Bernd Timmermann,  Erich E Wanker,  Sascha Sauer,  Ulrich Stelzl
Affiliations

Affiliation

  • 1 Otto-Warburg Laboratory, Max Planck Institute for Molecular Genetics (MPIMG), Berlin, Germany.
PMID: 23455924 DOI: 10.1038/nmeth.2397
Abstract

To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation Sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate Enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and validated through coimmunoprecipitation experiments and defines previously undiscovered cellular roles of nonhistone protein methylation.

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